color-dyed polystyrene nps  (PolyScience)

Journal: Drug delivery and translational research

Article Title: Individually coated microneedles for co-delivery of multiple compounds with different properties

doi: 10.1007/s13346-018-0549-x

Figure Lengend Snippet: Representative coated microneedle (MN) arrays. Photographic images of representative arrays of MNs (A) individually coated with five different-colored dyes and (B) all coated with the same dye. Coated dyes are 1) soluble crystal violet dye, 2) blue-dyed nanoparticles (NPs), 3) azure color from the mixture of blue-dyed and yellow-dyed NPs, 4) yellow-dyed NPs, and 5) red-dyed NPs.

Article Snippet: The coating solution also contained different dye species which were coated individually on MNs in designed combinations and sequences to generate the desired pattern on MN patches: (i) water-soluble small molecule drugs were simulated by either fluorescein sodium salt (Fluo) or sulforhodamine B (SRhB); (ii) water-insoluble small molecule drugs were simulated by oil blue N (OB); (iii) macromolecular or protein drugs were simulated by fluorescein isothiocyanate-labeled bovine serum albumin (BSA-FITC), which was synthesized as described previously [ 34 ] with modification (see Supplementary Information [SI] ); (iv) drug-loaded nanoparticles (NPs) were simulated by either color-dyed (red, blue, or yellow) polystyrene NPs (diameter 500 nm, Polybead, Polyscience, Warrington, PA), or fluorescent yellow-green polystyrene NPs (diameter 500 nm, Fluoresbrite, Polyscience, λ Ex /λ Em = 441/486 nm; see SI ).

Techniques:

Journal: Drug delivery and translational research

Article Title: Individually coated microneedles for co-delivery of multiple compounds with different properties

doi: 10.1007/s13346-018-0549-x

Figure Lengend Snippet: Co-delivery of free molecules and nanoparticles (NPs) in skin by a single microneedle (MN) patch. Schematic images showing the 5×5 pattern of MNs in a patch coated with (A1) free fluorescein sodium dye (Fluo) and fluorescently labeled nanoparticles (NPs), (C1) free sulforhodamine B dye (SRhB) and fluorescently labeled NPs, and (E1) free Fluo dye, free bovine serum albumin labeled with FITC (BSA sites) and fluorescently labeled NPs. Representative photographic images of porcine skin ex vivo (top view) showing the kinetic color change after applying a MN patch showing (A2–A6) the “emerging letter G” pattern, (C2–C6) the “disappearing letter π” pattern and (E2–E6) the disappearance of each of three different compounds over different time scales. The scale bar is 5 mm. Kinetic color change in panels (A, C, E) is quantified in panels (B, D, F), respectively. Data show mean ± S.D. (n = 4).

Article Snippet: The coating solution also contained different dye species which were coated individually on MNs in designed combinations and sequences to generate the desired pattern on MN patches: (i) water-soluble small molecule drugs were simulated by either fluorescein sodium salt (Fluo) or sulforhodamine B (SRhB); (ii) water-insoluble small molecule drugs were simulated by oil blue N (OB); (iii) macromolecular or protein drugs were simulated by fluorescein isothiocyanate-labeled bovine serum albumin (BSA-FITC), which was synthesized as described previously [ 34 ] with modification (see Supplementary Information [SI] ); (iv) drug-loaded nanoparticles (NPs) were simulated by either color-dyed (red, blue, or yellow) polystyrene NPs (diameter 500 nm, Polybead, Polyscience, Warrington, PA), or fluorescent yellow-green polystyrene NPs (diameter 500 nm, Fluoresbrite, Polyscience, λ Ex /λ Em = 441/486 nm; see SI ).

Techniques: Labeling, Ex Vivo